7 Steps to Prep Your Grain Spawn - Mushroom Media Online (2024)

Answers to the big questions about sterilization

One of the most important parts of your mushroom growing business is sterilization. When you are getting started, sterilization can be confusing. Do you use boiling water, cold pasteurization, pressure cookers (or canners), autoclaves (also referred to as sterilizers). Ultimately, what works for you will depend on your budget, farming set-up, and business needs.

Starting out you may purchase starter pressure cookers that may not get to the higher pressures possible with top end brands like the All American so you will have to run them longer, and you will not be able to sterilize as much spawn grain or substrate with each run. When you scale up your mushroom operation when the demand is there for larger sterilization runs, you will probably want to use a larger reliable brand of pressure cookers. We recommend the All American 941 model.

The All American 941 can hold 19 quart jars or 27 pint jars in each run. It can also hold up to 30 lbs. of substrate in Unicorn bags when stacked properly. The choice of a sterilizer or a pressure cooker often comes down to the cost of the unit. Pressure cookers tends to heat up faster than the electric sterilizers, especially the 110 versions. The 240 versions have more power and do heat up much faster than the 110. A good practice when you are doing runs in either a pressure cooker or the autoclave (sterilizer) is to reload the unit for the next run while it is still warm from the previous run. In order to do so, you will need tongs, or good gloves to deal with the heat on jars or sterilized bags.

7 Steps to Prep Your Grain Spawn - Mushroom Media Online (2024)

FAQs

How do you prepare grain for grain spawn? ›

Sterilize the grain: Rinse the grains and soak them in water overnight. Drain the water and fill the grains into jars or bags. Sterilize the grains in a pressure cooker or autoclave for at least 60 minutes at 15 PSI. We sell Small Autoclaves for Pressure Sterilizing grain spawn.

How do you make a mushroom spawn step by step? ›

How to Grow Oyster Mushroom Spawn (Low Tech)
  1. Step 1: Materials. ...
  2. Step 2: Prepare Clean Room. ...
  3. Step 3: Prepare Jars. ...
  4. Step 4: Prepare Grain. ...
  5. Step 5: Sterilisation. ...
  6. Step 6: Inoculation I (Grain Spawn Transfer) ...
  7. Step 7: Inoculation II (Agar Tissue Culture Transfer) ...
  8. Step 8: Inoculation III (Liquid Inoculation Methods)

How long to soak grain for mushroom spawn? ›

The first step to making grain spawn is to soak some cereal grain. Soak the grain by simply placing it in a bucket or bowl, covering in water, and leaving it for 12 -24 hours. If you leave the grain for too long, it will eventually sprout- so try not to soak it for more than 24 hours or so.

How wet should grain spawn be? ›

When ready, they should have no noticeable moisture on the outside of the grain. Pick up a handful of grains to test. They should be loose individuals kernels, dry on the outside, and swollen with water on the inside.

How to sterilize grain spawn without a pressure cooker? ›

First, fill the jars with your grain and water mixture, but leave the lid slightly loose to allow steam to escape. Microwave on high for 10-15 minutes, or until the grain has been sterilized. Be sure to let the jars cool down before tightening the lid and inoculating.

What is the process of spawn preparation? ›

In the spawn-production process, mycelium from a mushroom culture is placed onto steam-sterilized grain, and in time the mycelium completely grows through the grain. This grain/mycelium mixture is called spawn, and spawn is used to "seed" mushroom compost.

What is the best grain for mushroom spawn? ›

When making grain spawn, the gold standard has always been rye grain. And that makes sense. Rye is widely available, hydrates well- and is universally loved by almost any mushroom species.

How long does it take for mycelium to grow on grain? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions.

Can you speed up mycelium spreading? ›

Mycelium growth can be sped up by optimizing certain conditions. Here are some key factors: 1. Temperature: The optimal temperature for mycelium growth is usually between 24-27°C.

What makes mycelium grow faster? ›

The higher the inoculation rate or amount of spawn added to the substrate, the faster the mycelium will grow through the substrate. The substrate likely will not produce a larger amount of mushrooms, so a high inoculation rate means more money spent on spawn per pound of mushrooms.

What is the best temperature for mushroom grain spawn? ›

The ideal temperature for a spawn run depends on the type of mushroom, but it is generally between 23 and 28 Celsius (73 and 82 Fahrenheit).

How do you make perfect grain spawn? ›

How to Make Grain Spawn Step-by-Step
  1. Hydrate The Grain. The first step in hydrating your cereal grain is to soak the grain in water. ...
  2. Drain The Grain. ...
  3. Fill Your Jars. ...
  4. Sterilize The Grain. ...
  5. Inoculate The Grain. ...
  6. Incubate The Grain Spawn.

What size bags for grain spawn? ›

In my experience, the large sized bags are by far the most useful. To give you an idea, a bag of size 8 inches wide by 5 inches deep by 18 inches tall will comfortably hold 5 lbs of supplemented sawdust and grain spawn.

How to tell when a spawn bag is ready? ›

Storage & Colonization
  1. Allow your bag to colonize undisturbed and out of direct sunlight. ...
  2. After about 4-8 weeks, you should start to see mycelium growing on the substrate. ...
  3. Your spawn is ready to use when the bag is completely white with mycelium.

How do you prepare grains? ›

Plain Grains, general directions

Cooking most grains is very similar to cooking rice. You put the dry grain in a pan with water or broth, bring it to a boil, then simmer until the liquid is absorbed. Pasta is generally cooked in a larger amount of water; the excess is drained away after cooking. Don't be intimidated!

What is the best grain for grain spawn? ›

Rye. Many people consider rye to be the best grain for mushroom spawn.

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