Demystifying rennet, a key ingredient in cheesemaking (2024)

We all know the main ingredient in cheese is milk (and we spend a lot of our time talking about it), but what else is in cheese? Most cheeses are comprised of milk, salt, cultures and rennet. Rennet is the most fascinating of these ingredients, yet it is a mystery to many.

Although the origins of cheesemaking are murky and still much debated, one of my favorite “origin tales” is that of the nomadic shepherd who was traveling with milk in a sack made from the stomach of a young animal. Upon arriving at his destination, the shepherd went to take a swig of milk only to find that it had curdled into a delicious solid. While this is probably just a fable, it still speaks to a huge discovery in the science of cheesemaking! Something magical happens to milk in the stomachs of young ruminants that helps cheesemakers achieve our primary objective: to extend milk’s “shelf life”. Rennet is the magic!

But first, a little science primer. Milk is an emulsion of fat, protein, sugar and minerals suspended in water. Cheesemakers use one of a few different methods to trigger a chemical reaction that causes coagulation, transforming liquid milk into a solid coagulum, or curd. Coagulation occurs when proteins clump together, trapping fat and other solids like a sponge. For cheddar, a hard aged cheese, we use enzymatic coagulation--by adding rennet. For more on other methods of coagulation, check out this great article on Cheese Science Toolkit

Phew! Now that some of the chemistry is out of the way, what exactly is rennet? And where did it come from? Rennet is the general name for enzymes that act on proteins in milk. It’s purpose in a ruminant’s stomach is to curdle milk for easier digestion, the same way it curdled our shepherd’s drink. Rennet serves the same purpose in cheesemaking: it triggers coagulation.

Demystifying rennet, a key ingredient in cheesemaking (2)

Demystifying rennet, a key ingredient in cheesemaking (3)

Demystifying rennet, a key ingredient in cheesemaking (4)

There are several types of rennet. At Shelburne Farms, we utilize traditional calf rennet. Calf rennet contains two enzymes, chymosin and pepsin, in a natural ratio that is suitable for our style of cheese. (Chymosin causes coagulation, pepsin has impacts on the aging process.) This traditional rennet has more reliable results in yield, as well as flavor profile, leading to less defective cheese. It is also a natural and non-GMO option that exemplifies our efforts to be sustainable by utilizing all parts of the animal.

In some cultures, vegetable and plant rennet has been used to coagulate milk. In India, where the cow is revered for religious reasons, fruit trees were used. Many Spanish sheeps’ milk cheeses use thistle and cardoon rennets. One drawback of these coagulants is they may lead to bitter and undesirable flavors in cows’ milk cheeses.

A demand for an inexpensive and reliable alternative to calf rennet led to the development of a genetically modified bacterium that can produce rennet enzymes through fermentation, calledfermentation produced chymosin. In 1990, Pfizer successfully produced a chymosin that is genetically identical to the chymosin found in calf rennet. It is filtered from the bacteria in which it’s grown and isolated. Fermentation produced chymosin does not contain pepsin, so for our purposes, it does not have the exact same impact on the aging process.

Calf rennet is now the only rennet we use here as it is best for the flavor profile we are seeking. We haveused fermentation produced chymosin at Shelburne Farms in the past, and we believe the product is safe and reliable, and can be part of a healthy food system. Debate on whether these enzymes are effectively a genetically modified organism is ongoing. As of 2008, according to the culture houses who produce these products, 80-90 percent of cheeses in the United States and Britain utilize fermentation-produced chymosin. At the same time, it has been banned in several countries that do not allow genetic engineering of food products.

I hope this introduction to rennet helps to demystify this important cheese ingredient. As with all our food choices, understanding how a product is made can help us make more informed decisions. Still, the world of rennet is particularly complicated, both scientifically and chemically, and it raises important questions around food system health. For small cheese makers in Vermont, the choice of which rennet to use is not a simple one.

If you want to dive even deeper into this topic, check out the entry on “rennet” in the Oxford Companion to Cheese, edited by UVM professor Catherine Donnelly, with a forward by Jasper Hill Farm founder Mateo Kehler. UVM professor Paul Kindstedt has written two definitive books on cheese that cover rennet at length, American Farmstead Cheese and Cheese and Culture. (You can find copies at our Welcome Center and Farm Store.)

Demystifying rennet, a key ingredient in cheesemaking (2024)

FAQs

Demystifying rennet, a key ingredient in cheesemaking? ›

Rennet is the general name for enzymes that act on proteins in milk. It's purpose in a ruminant's stomach is to curdle milk for easier digestion, the same way it curdled our shepherd's drink. Rennet serves the same purpose in cheesemaking: it triggers coagulation.

What does rennet do in cheese making? ›

One of the essential ingredients in making nearly every kind of cheese, rennet is a set of enzymes that cheesemakers use to coagulate milk and separate solid curd from liquid whey.

What happens if you use too much rennet in cheese making? ›

Too much rennet was used or too much butterfat, left your cheese during the process. Rubbery cheese can happen when an excess amount of rennet is used, too much rennet equals a rubber ball, too little, soup!

What 2 things does rennet create when introduced to milk? ›

Curds and Whey

When we make cheese, our goal is to separate the solid components of milk (casein protein, fat, minerals) into curds and the liquid into whey (water, lactose, whey protein). This separation is possible due to coagulation and usually rennet is the enzyme used to achieve this.

What cheese is made with rennet? ›

Most hard cheeses, including Parmesan, Cheddar, Manchego, Pecorino Romano, and Swiss, are traditionally made with rennet, while some soft cheeses aren't (scroll down for five you can try). But increasingly, you can find all sorts of cheeses made with non-animal-derived enzymes.

What is the best rennet for cheese making? ›

Calf rennet contains two enzymes, chymosin and pepsin, in a natural ratio that is suitable for our style of cheese. (Chymosin causes coagulation, pepsin has impacts on the aging process.) This traditional rennet has more reliable results in yield, as well as flavor profile, leading to less defective cheese.

Can cheese be made without rennet? ›

Younger, soft cheeses like mozzarella, cottage cheese, cream cheese, ricotta, goat, and provolone often are not made with rennet. However, this isn't an exhaustive list, so be sure to read your labels carefully!.

What temperature kills rennet? ›

Rennet is an enzyme used to coagulate milk, in order to form a thick curd. Rennet begins working at temperatures between 85-105F, even at higher temperatures it will not be deactivated until it reaches 140F.

Is rennet bad for you? ›

Most folks are ok with traditional rennet, which is derived from the stomach lining of animals (remember – no animal is ever sacrificed only for rennet). However, some choose to stick with “vegetarian” cheeses, which use plant-based or synthetic rennet to coagulate the milk.

Does rennet expire? ›

Liquid rennet (both animal and vegetable types) should be stored in the refrigerator but should never be frozen. Liquid vegetable rennet has a recommended shelf life of about one year. Dry rennet varieties should also be refrigerated when stored and have a longer shelf life compared to their liquid counterparts.

Are calves killed for rennet? ›

Calf rennet is extracted from the inner mucosa of the fourth stomach chamber (the abomasum) of young, nursing calves as part of livestock butchering. These stomachs are a byproduct of veal production.

Does tillamook cheese use Pfizer rennet? ›

The exact method of making this fermentation-produced rennet is proprietary. However, it is important to note that our rennet is classified as “not genetically engineered” by USDA bioengineered food labeling regulations. Furthermore, we do not use rennet that is produced by any pharmaceutical company.

Does rennet need to be refrigerated? ›

Rennet has a limited shelf life – liquid rennet lasts for 7-8 months when refrigerated and tablets last for up to 5 years when stored in the freezer (2 years at room temp). If you are unsure how old your rennet is or whether or not it is still effective, you can do the following test: Heat one cup of milk to 90F.

Does all mozzarella have rennet? ›

If you're wondering does mozzarella cheese have Rennet, the answer is both yes and no. You can pick an animal-based or plant-based mozzarella. In contrast, rennet-free mozzarella cheese has a mild flavor and a soft texture ideal for creating panini or pizzas.

Does brie contain rennet? ›

Brie is made with rennet and warmed up to 37º Celsius to obtain the curd and most of its flavor. Once the perfect texture is achieved, it's ladled into the round molds where it sits for up to 18 hours. Then, the cheese is carefully salted before it's aged.

Does all Parmesan have rennet? ›

“Calf stomachs are ground and then soaked in an enzyme extraction solution,” which makes the end products not vegetarian by most standards. While all cheeses labeled Parmigiano-Reggiano definitely contain animal-derived rennet, Parmesan can go either way.

Does rennet require slaughter? ›

The reason why animal rennet is not a vegetarian substance is that while it is derived from animals, it cannot be extracted from live animals. The animals involved in producing animal rennet need to be slaughtered before their stomachs can be used to harvest rennet, which obviously makes it not vegetarian.

What to use instead of rennet? ›

Pig pepsin and chicken pepsin were used previously, however, not now. All successful rennet substitutes are aspartyl proteinases. Proteinases from Rhizomucor miehei, R. pusillus, Cryphonectria parasitica, Aspergillus oryzae and Irpex lactis are used for commercial cheese production.

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