Inoculation of substrate bags with spore syringes - Tyroler Glückspilze (2024)

FAQs

How do you inoculate substrate with spores syringe? ›

Inoculation: Shake the syringe to dispense the spores equally. Now put the needle into the bag (at the disinfected area, always above substrate level) and push the spore solution of the same species into the bag. We recommend to use about 10 -‐ 20 ml – that is 1 -‐ 2 syringes -‐ for small bags (2,5 L content).

After inoculation, the spawn needs time to grow through the substrate. This process is called incubation. Incubation starts with leap off, which is the moment when the mycelium initially leaps off the spawn onto the new substrate. This typically occurs around four days after inoculation.

Stab the inoculation tool into the spawn a couple of times, filling the chamber with sawdust. Then place the inoculator over the hole in the log and eject the spawn into the hole. The spawn should be tightly packed into the hole and rest approximately 1/8 inch below the surface of the log.

Storage & Colonization

Keeping your bag at the higher end of the temperature range will encourage faster colonization. 2. After about 4-8 weeks, you should start to see mycelium growing on the substrate.

You can tell when mycelium is fully colonized when the entire substrate (such as a jar or a bag) is covered with a dense, white, and fuzzy network of mycelial threads. There should be no visible uncolonized areas or contaminants, and the mycelium should appear healthy and vigorous.

Radial growth of the mycelium was faster under dark incubation rather than under light incubation.

Mycelium growth can be sped up by optimizing certain conditions. Here are some key factors: 1. Temperature: The optimal temperature for mycelium growth is usually between 24-27°C.

Keep your spawn bags in a warm, dark spot (75-77 degrees optimally) and try to leave it alone! Spawn bags colonize from the inside out so you will most likely won't see any mycelium growth for 7-14 days.

begins, you should see white growth within 5-7 days and should quickly spread and fully colonize in anywhere from 14-21 days. After the jar is fully colonized, its best to wait and extra 2-3 days to make sure the center is also finished.

Without fresh air, the mushroom culture would eventually stop growing, but if the bag were just left open after sterilization, contaminates would quickly take over. The filter patch is the perfect solution.

Can spore syringe turn into liquid culture? ›

If you have a spore syringe but would rather work with culture, you can use our Liquid Culture Kit to turn your spore syringe into liquid culture. You can also use the Liquid Culture Kit to expand a liquid culture syringe into much more liquid culture.

You can also inoculate sterilized grain from a syringe- either a liquid culture or a spore syringe. In order to do this, you first need to flame sterilize the tip of the syringe until it is red hot. Then you can quickly lift the lid of the grain jar and inject the syringe.

The best time to inoculate logs is in spring, within one to two weeks after the logs have been cut. This allows the cells in the tree to die but is not long enough for the log to dry out or for other competitor fungi to become established. Logs can be inoculated with either the drilling method or the totem method.

The answer is no. The window of inoculating hardwood logs is anywhere from 1 week to 6 weeks after cutting. In cooler climates, some growers cut logs in the fall, stack, and cover them with a tarp to overwinter, then inoculate in the spring.

One syringe has 20 ml of the mushroom spore solution which is enough to inoculate between 6 and 7 liters of substrate. Drop a few milliliters in each corner of the substrate and everything is set. After some days, the growth of white patches of mycelium can be noticed on the spot where the mushroom spores were dropped.

Mushroom spores suspended in sterile water inside of a syringe are called spore syringes. Both can be used to cultivate mushrooms by injecting a portion of the syringe into a sterilized grain bag which colonizes and can then be transferred to sterile substrates such as manure, straw, or sawdust.

Inoculate Your Culture: After cooling, introduce spores or mycelium from a healthy culture into your sterile liquid medium. This step kickstarts the mycelial growth – feel free to offer some words of encouragement to your culture for good measure. Gently Mix: Shake the jar softly to evenly distribute the mycelium.