Understanding the Best Grain for Mushroom Spawn Propagation  (2024)

Understanding the Best Grain for Mushroom Spawn Propagation (1)

Today we are discussing some of the best grain for mushroom spawn creation and the mushroom strains that thrive as grain spawn

People often wonder about the best grain for mushroom spawn. Grain is great because it allows for advantageous spawn. It is also a great option for supplementing other growing mediums.

Grain spawn gives one major advantage by supplying a pre-colonized Nitrogen source to the substrate. This can help to increase yields of low nitrogen substrates like straw or paper waste. If you peruse the internet for information on grain spawn, as we do frequently, you will likely see that rye is considered to be the gold standard in growing mushrooms on grain. However, there are some that believe millet is the best grain for mushroom spawn. The reason: Smaller grains like millet are preferred to larger grains like rye or wheat as they allow for more inoculation points from the same amount of inoculum. One pound of rye spawn may have about 200 particles for the mycelium to leap off from while millet will have closer to 500. By using millet, a smaller grain, the mycelium will spread more evenly and abundantly through the substrate. Similarly, sawdust can be better than larger grains because of the small particle size.

The use of grain in growing mushrooms

Grain is used as a high-nutrient medium for rapid mycelial expansion. It is typically the step after mycelial growth on agar medium. Grain is too expensive and too high in nutrients for bulk substrate fruiting, but can be a good supplement to the sawdust or wood pellet base. It works well to boost yields by inoculating straw with large amounts of grain spawn. Grain also needs to be treated using pressurized steam and inoculated in lab conditions. It is very easy to get contamination on grain, so this is not a beginner substrate.

Supplementing sawdust with the use of grain

Grain spawn is typically a lower generation and has a higher nutrient profile. This makes grain spawn a good option when the substrate will be further expanded or if you want to add additional nutrients into the substrate. For example, if inoculating grain with the intent of then adding it to a sawdust fruiting block, you would want to use a first or second generation bag of grain spawn. If you were inoculating straw or supplemented sawdust, using grain could be a good option for adding additional nutrients into the substrate. One advantage of using sawdust in this case is that the mycelium is already familiar with eating sawdust, so leaping off might be faster off sawdust than grain.

Understanding the “generations” of grain spawn

The generations of spawn are very straight forward. The first substrate inoculated from a petri plate is usually grain and called Generation 1. This is then expanded 8-10x into more grain which is Generation 2 grain. From here, farmers usually either go to Generation 3 grain or sawdust. Most growers do not perform transfers beyond four generations for fear that the strain losing vigor in fruiting -- a phenomenon called strain senescence.

Types and strains of mushrooms that grow well on grain

Grain spawn is a good option for indoor growing. We’re going to take a look into various mushrooms and strains of those mushrooms to use for yielding tasty mushrooms indoors, at home or your commercial mushroom space.

For shiitake lovers there is the Shiitake Grain Spawn LE 46. This strain is known as the workhorse for commercial mushroom farmers who use logs. If you are looking for big yields from a strain that colonizes quickly, then this is a great option for you. The fruited mushrooms themselves are beautiful, presenting a dark cap with white ornamentation of its edges. If you are using hardwood logs for cultivation then this is another great reason to use this strain. This mushroom loves hardwood, fruits at a wide range of temperatures, and responds well to shocking. A log farmer’s dream! You can buy your own Shiitake Grain Spawn LE 46 here now.

Next we will consider the Blue Oyster Grain Spawn 3015. Do you want to try your hand at growing oyster mushrooms indoors on straw, wood, or used coffee grounds? If you do and you want great yields, then this is the strain to try. Oyster mushrooms are a perfect introduction to mushroom growing for beginners because they are one of the easier types of mushrooms to grow. Fortunately, they are also delicious and pleasant to look at as they fruit. This blue oyster grain spawn strain produces large fruiting bodies, has a fast spawn run, and yields mushrooms with a beautiful blue color that is vibrant when young. This mushroom fruits in a wide range of temperatures between 55 and 75 degrees Fahrenheit.

You can buy your own Blue Oyster Grain Spawn 3015 here now. Try these strains and any other of our grain spawn options and let us know what you think the best grain for mushroom spawn is.

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Understanding the Best Grain for Mushroom Spawn Propagation  (2024)

FAQs

What is the best grain for mushroom grain spawn? ›

Without a doubt, however, the most common grain used for mushroom cultivation is Rye. This is because of Rye's water absorbing qualities, nutrient makeup, and, well, mushrooms seem to like it.

How to make perfect grain spawn? ›

  1. Step 1: Hydrate The Grain. The first step to making grain spawn is to soak some cereal grain. ...
  2. Step 2: Making The Lids. While your grain is soaking, it's a perfect time to make your specialized lids. ...
  3. Step 3: Simmer, Drain and Dry. ...
  4. Step 4: Sterilize. ...
  5. Step 5: Inoculate. ...
  6. Step 6: Colonize.

What is the grain to substrate ratio for cubensis? ›

It's important to maintain a spawn to substrate ratio of 1:3 or 1:4 for cubensis and b plus mushrooms and ensure proper sterilization or pasteurization of the mushroom substrate before inoculation to prevent contamination.

What makes mycelium grow faster? ›

The higher the inoculation rate or amount of spawn added to the substrate, the faster the mycelium will grow through the substrate. The substrate likely will not produce a larger amount of mushrooms, so a high inoculation rate means more money spent on spawn per pound of mushrooms.

What is the best temperature for mushroom grain spawn? ›

The ideal temperature for a spawn run depends on the type of mushroom, but it is generally between 23 and 28 Celsius (73 and 82 Fahrenheit).

How long does mycelium take to grow in grain spawn? ›

Colonization occurs after inoculation. This is when the mycelium will start to take over the grain that was inoculated with spores. This period usually lasts between 3 to 6 weeks depending on strain and environmental conditions. Ideal conditions for this phase are in a dark place and temperature between 72 F – 80 F.

How wet should grain spawn be? ›

Then remove the grains and look for wet spots on the paper towel. If there's a damp spot, your grains are still too wet. The paper towel should be dry. Once you can complete this test, it's safe to load them in jars or bags for sterilization.

How many times can you multiply grain spawn? ›

Grain Spawn is meant for folks with a little more advanced cultivation skills. Grain is a wonderful substrate because you can expand it 10 times, then expand each 2nd generation spawn jar 10 times - one jar can then make 100 jars of spawn!

Can I refrigerate colonized grain spawn? ›

For longer storage, refrigerate the spawn for up to 3 months. If you use a partial bag of spawn, store the remaining spawn in its original bag, folding the top down from the filter patch and clipping to secure. Pink Oyster grain spawn CANNOT be refrigerated and should be used within two weeks of receiving.

What is the best humidity for cubensis mycelium growth? ›

The growth environment (i.e., temperature, relative humidity, access to oxygen, clean/ventilated air exchange, and lighting conditions) also impacts mycelium growth. The temperature should be kept at around 25–30 °C [7,13,34] and the relative humidity around 70–80% [7].

How much coffee grounds to add to mushroom substrate? ›

Coffee grounds should comprise 77% of your substrate's total weight. Pasteurized straw or sawdust equal to 15% of the total substrate weight or 20% of the weight of the coffee grounds. Oyster or sh*take mushroom spawn equal to 8% of the total substrate weight or 10% of the weight of coffee grounds.

What is the best ratio of spawn to substrate? ›

Break up and mix spawn, adding it at a rate of ½ bag to 1 full bag of spawn per 5 gallons of substrate. A higher ratio of spawn leads to faster and more reliable colonization and is recommended for beginner growers.

Does mycelium grow faster in the dark? ›

Mycelium grows just fine in a dark place, and some daylight does not matter. Do not place the mycelium in direct sunlight, this will heat the substrate and it could dry out faster.

How can I make mycelium spread faster? ›

Light: While mycelium doesn't require light to grow, a small amount can help stimulate growth. 5. Nutrition: Using a nutrient-rich substrate can also enhance growth. Remember, it's important to maintain sterile conditions to prevent contamination.

What speeds up mushroom growth? ›

Gypsum is a mineral that helps speed up the mushroom growing process in small amounts.

What is the grain spawn to substrate ratio for a monotub? ›

Spawn and bulk substrate should be mixed at a ratio if 1 parts spawn to 2/3 parts bulk substrate. If you have to much bulk substrate it could take to long to colonise and contamination could set in. Say you have 1l of spawn and you mix it with 2l ob bulk you would have a total of 3l to spawn to your tub.

Can you use rice for grain spawn? ›

A variety of grains can be used to make good grain spawn. Most commonly used are rye, millet, brown rice, corn, and more. When choosing which grain to use its important to keep various factors in mind. Grain size, price, and quality are the most important factors to consider.

Can I use barley for grain spawn? ›

Grains including brown rice, rye berries, wheat berries, millet, barley, oats and even corn are used to make grain spawn. In selecting grain for this purpose it is important to note that grain size factors into colonization speed, as smaller grains have more inoculation points and mix more evenly into a bulk substrate.

What is mushroom grain spawn made of? ›

Grain Spawn is mushroom mycelium grown into certified organic rye or millet grain which is ideal for inoculating sterilized or pasteurized substrates. Grain spawn is also the industry standard for high volume commercial production.

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