Collecting a urine sample (2024)

Collecting a urine sample (1)

https://www.gosh.nhs.uk/conditions-and-treatments/procedures-and-treatments/collecting-urine-sample/

Urine is a waste product produced by the kidneys, so testing it can show if the kidneys are not working well. Testing a urine sample is usually the first stage in carrying out a general assessment, before blood tests are needed. This information sheet from Great Ormond Street Hospital (GOSH) explains how to collect a clean urine sample for testing.

Make sure you do not touch the inside of the urine sample bottle or test tube or the top edge – you may introduce bacteria which could contaminate the sample.

Only use the urine sample bottle or test tube provided – if you have not been given one, ask at your family doctor (GP) surgery.

The bottle or test tube does not need to be completely full – usually a small sample is enough for testing.

Collecting a sample from children and young people who are potty or toilet trained

You will need:

  • Clean container or foil bowl
  • Urine sample bottle or test tube as provided
  • Clean funnel or oral syringe (if required)

What to do:

  1. Wash the genital area with water and pat dry with a clean towel
  2. Wash your hands with soap and water
  3. Ask your child to start weeing and then use the container to catch their wee
  4. Do not collect the first few drops – wait a second or two before starting to collect
  5. Pour the sample from the container into the urine sample bottle (using a funnel or syringe if needed)
  6. Screw the lid on tightly and put in the biological hazard bag you have been given then give to your doctor

Collecting a sample from babies and children in nappies or pads using cotton wool or gauze

You will need:

  • Cotton wool or gauze
  • Syringe
  • Urine sample bottle or test tube as provided

What to do:

  1. Wash the genital area with water and pat dry with a clean towel
  2. Wash your hands with soap and water
  3. Put a thick layer of cotton wool or gauze inside your child’s nappy
  4. Wait 10 minutes and check whether it is wet
  5. If it is wet, use the syringe provided to draw up the urine to put in the urine sample bottle or test tube.
  6. Screw the lid on tightly and put in the biological hazard bag you have been given then give to your doctor
  7. If it not wet, replace the nappy and wait another 10 minutes before checking. Replace the pad every 30 minutes to avoid contamination.

Using a urine collection bag

You will need:

  • Urine collection bags
  • Urine sample bottle or test tube as provided

What to do:

  1. Wash the genital area and pat dry with a clean towel
  2. Wash your hands with soap and water
  3. Attach the bag around your child’s genitals using the sticky strip
  4. Check the bag regularly and remove when your child has weed.
  5. Cut the corner off the bag and pour urine into the urine sample bottle or test tube.
  6. Screw the lid on tightly and put in the biological hazard bag you have been given then give to your doctor or nurse.

When do we get the results?

Quite often, the doctor or nurse will test the urine straightaway using a testing strip. This contains chemicals that react with the urine.

  • If there is protein in the urine sample, the kidneys may not be filtering it out well.
  • If there is sugar in the urine sample, this might be an indicator that sugar levels in the blood are too high.
  • If your child has a urine infection, the bacteria may be present in the urine sample.

Once the doctor or nurse has dipped the strip into the urine sample, they wait a few seconds until the colours develop and then compare it with the colours on the bottle to get the results.

Occasionally, particularly if an infection has been identified, the doctor or nurse may send the sample off to the laboratory for testing. The laboratory will grow the bacteria in the sample to try to work out the particular type. This means that any antibiotic your child has will work specifically against the bacteria present.

  • If you have any questions,please ask your doctor or nurse.

Compiled by:

The Urodynamics department in collaboration with the Child and Family Information Group

Last review date:

June 2020

Ref:

2020F1908

Disclaimer

Please note this is a generic GOSH information sheet. If you have specific questions about how this relates to your child, please ask your doctor. Please note this information may not necessarily reflect treatment at other hospitals.

Useful documents

  • Download Collecting a urine sample F1908 FINAL Jun20.pdf (0bytes)

  • Download Giving a wee sample ER0063 FINAL Jun20.pdf (306.5KB)

Collecting a urine sample (2024)

FAQs

Collecting a urine sample? ›

Urinate a small amount into the toilet bowl, and then stop the flow of urine. Then collect a sample of urine into the clean or sterile cup, until it is half full. You may finish urinating into the toilet bowl.

What is the proper way of collecting a urine specimen? ›

Urinate a small amount into the toilet bowl, and then stop the flow of urine. Then collect a sample of urine into the clean or sterile cup, until it is half full. You may finish urinating into the toilet bowl.

How fresh does a urine sample need to be for testing? ›

The sooner the sample is given in to the doctor's surgery, or to the laboratory, the better. Within two hours is best. If that is not possible, put the sample in the fridge until you take it to the doctor or laboratory.

What is the best way to take a urine sample? ›

Start urinating into the toilet. After the first part of the urine has gone into the toilet, put the clean bowl or pot under the stream of urine. Catch some urine in the pot/bowl (1/2 full is fine). Then remove the bowl/pot.

When is the best time to collect urine sample? ›

It is preferred that you collect a specimen first thing in the morning, because the first morning urination is more concentrated and likely to yield the best results. Prior to urinating, it's important to wipe your genital area clean so that the specimen is not contaminated with bacteria from your skin.

Why is early morning urine the best sample to be tested? ›

Your doctor may, for example, request a first morning sample because the urine is more concentrated and therefore it is more likely to show up any abnormalities. Or if the doctor is looking for glucose in the urine they may ask you to collect a sample after a meal.

What happens if you don't wipe before a urine test? ›

Cleansing the labial area prior to urination (clean catch collection) will remove most of the organisms from the labia and the urethral opening thereby increasing the probability of a false negative result.

What will mess up a urine test? ›

Clinically, a false positive urine drug screen can be due to numerous xenobiotics: dextromethorphan, diphenhydramine, doxylamine, ibuprofen, imipramine, ketamine, meperidine, venlafaxine, buproprion, methylenedioxpyrolvalerone (MDPV), and tramadol.

What should you avoid before a urine sample? ›

Patient must abstain from avocados, bananas, tomatoes, plums, eggplant, hickory nuts, walnuts, pineapple, coffee and mollusks. and during collection: aspirin, corticotropins, MAO inhibitors, phenacetin, catecholamines, reserpine and nicotine. Restrict caffeine, nicotine and alcohol 24 hours prior to collection.

Should you drink water before a urine sample? ›

Before your urinalysis test, you'll need to make sure you've drunk enough liquids, such as water, to be able to go to the bathroom and produce a urine sample. Depending on the reason for the urinalysis, your healthcare provider may want the urine sample to be the first time you pee in the morning (first morning void).

How do I get good urine test results? ›

Before collecting a sample, it's a good idea to cleanse your genital area with water (no soap). Otherwise, the urine may be contaminated with bacteria, cells or other substances. To get an accurate result and avoid bacterial contamination, “clean” midstream urine is used.

How do you collect a urine sample from an elderly person? ›

If the person is incontinent and wears incontinence pads, a urine collection pack can be used to draw a sample of urine from the pad. These are usually made up of two urine collection pads, a 5ml syringe and a urine specimen container.

How can I get a urine sample faster? ›

If you do have to force yourself, here are 10 strategies that may work:
  1. Run the water. Turn on the faucet in your sink. ...
  2. Rinse your perineum. ...
  3. Hold your hands in warm or cold water. ...
  4. Go for a walk. ...
  5. Sniff peppermint oil. ...
  6. Bend forward. ...
  7. Try the Valsalva maneuver. ...
  8. Try the subrapubic tap.

How to properly collect a urine sample? ›

Please follow these instructions:
  1. Clean genital area with the wipes. ...
  2. Allow first urine flow to go into the commode.
  3. Catch the "mid-stream" of the urine in the sterile container.
  4. Replace lid firmly on the container.
  5. Label specimen with name, date and time of collection.
  6. Place the specimen in the transport bag.

What is the most common mistake in the 24-hour urine collection? ›

Forgetting to collect some of your urine. Going beyond the 24-hour collection period and collecting too much urine. Losing urine from the specimen container through spilling.

How long should you not pee before a urine sample? ›

The recommended time to take a urine sample is as soon as you wake up, i.e. urine after an overnight 8-hour rest or if it is impossible, after at least 4 hours from the previous urination.

What is the proper way to collect a urine sample for culture quizlet? ›

  1. clean perineal.
  2. start urinating to toilet.
  3. midstream is the MOST sterile urine ( so collect this urine ) - Do not catch the first stream of urine.
  4. label it.

What is the best way to collect a urine sample for culture? ›

A small amount of urine should initially fall into the toilet bowl before it is collected (this clears the urethra of contaminants). Then, in a clean container, catch about 1 to 2 ounces of urine and remove the container from the urine stream. The container is then given to the health care provider.

How should urine be collected for urinalysis test? ›

What you can expect
  1. Cleanse the urinary opening. ...
  2. Begin to urinate into the toilet.
  3. Pass the collection container into your urine stream.
  4. Urinate at least 1 to 2 ounces (30 to 60 milliliters) into the collection container.
  5. Finish urinating into the toilet.
  6. Deliver the sample as directed by your health care provider.
Oct 24, 2023

What is the appropriate way to collect a urine specimen from a catheter? ›

A catheter specimen must be obtained from the sampling port on the catheter bag. Sampling ports are designed to be accessed directly using a Luer Lock syringe and do not require a needle.

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