How to identify contamination in mushroom growing (2024)

When learning to grow mushrooms you're almost guaranteed to encounter some other fungi in the form of moulds. Moulds are the biggest competitor to the mushroom grower. How do you identify it and what do you do when you find it?

Unfortunately the ideal growing conditions for mushrooms are also inviting to other fungi, including moulds - yuck!

Below we'll outline some common moulds that you may encounter while mushroom growing and what to do if you see some.

If you're growing a Mushroom Grow Kit purchased from us and it has developed some mould pleasesend us a message and we'll help you out in no time!

**We do not recommend eating mushrooms that you have grown that are touching mould**

Types of mould

Trichoderma (green mould)


A mushroom grower's enemy #1 - Trichoderma is a genus of fungi which contains over 80 accepted species. Trichoderma mould is usually green in colour, ranging from an olive to emerald green. It is present in all soils and is the most common contaminant found in mushroom growing.

Have you ever found mould on your onions or your bread when the weather gets warm and humid? That's Trichoderma.

It's fast growing and likes temperatures between 25-30 degrees Celcius so you'll more often encounter it in summer and it can take a hold before your mushrooms have had a chance to grow.

What it looks like

Ranges in colour from pale green, olive and dark emerald green. It can also appear yellow and usually has a white leading edge and green centre.

How to identify contamination in mushroom growing (1)

Far left image:A small patch of Trichoderma being surrounded my white mushroom mycelium attempting to contain the spread. This could be removed.

Centre image:A tiny patch of Trichoderma which has developed in the cut area. Mushrooms will grow around it. Spray it with isopropyl alcohol (be careful not to spray your mushrooms) or remove the small patch and the mushrooms will finish growing before it spreads further.

Far right image: A large patch of Trichoderma which has taken over the mycelium. Mushrooms won't grow and it's best to abandon this block.

What to do about it

If you encounter Trichoderma mould it if will effect the mushroom's ability to grow the best crop possible but it doesn't mean it will not grow at all.

If it's a small patch but mushrooms are already growing:

If the mould area is only small, smaller than a 50 cent coin for example, the mycelium may contain it itself or you can remove it.

  1. Ideally, if you have it available, spray the small patch of mould with isopropyl alcohol (rubbing alcohol) to kill it before removing it. If you don't have isopropyl alcohol, don't worry, just be careful not to touch it or disturb it as it may release spores. You can place a small bit of wet paper towel over the patch as you remove it to stop the spores releasing into the air.
  2. Cut it out of the block or scoop the patch out with a clean knife or spoon. Make sure to take an area 1-2cm from the edge of the patch to capture any mould that could be developing outwards from the edge.
  3. Once you've removed the mould patch continue misting your fruiting block as normal.

If it's a small patch but mushrooms haven't appeared yet:

If a patch of mould has developed on the surface but no mushrooms have grown yet the mushrooms will not appear where the mould has formed and may not grow from that side.

  1. Tape up the cut area of the fruiting block being careful not to disturb the mould or you can remove the patch as per instructions above.
  2. Turn the block around and see what the other side of the fruiting block looks like. If it's got strong white mycelium, cut an X on the fresh side and start from that side.
  3. If it doesn't grow on the other side or if more mould develops you will have to abandon this attempt.

If it's mouldy on half or more of the block:

  1. Remove the substrate block from the bag and bury it in your garden or compost it. Trichoderma is a soil fungi and can help to prevent other fungal diseases in the roots of your plants.
  2. If you don't have somewhere to bury it, dispose of the block in the bin.

Black mould


Very rarely you might encounter black mould. This mould can effect people with allergies, asthma or breathing difficulties and you should remove a fruiting block contaminated with black mould immediately from your home.

It is slow growing and thrives in constantly wet environments.

If you are over-watering your mushroom block you might leave it exposed to black mould.

What it looks like

Dark black and moist.

How to identify contamination in mushroom growing (2)What to do about it

If you encounter any black mould dispose of the mushroom block immediately. Mushrooms will not grow from a block which has become contaminated with black mould.

Neurospora crassa (orange bread mould)


This mould is extremely common in nature. Due to its ease to grow it has been used for decades in laboratory research into filamentous fungi. Its entire genome has been mapped and it is highly researched.

Unfortunately in mushroom growing circles it's got another name...farm killer. It's speed of growth and proliferation means it can easily overwhelm your mushroom farm and can be hard to eradicate. If you're growing commercially and find this in your farm take immediate action.

It's mainly present in tropical and sub-tropical regions and you might encounter it more in the warmer months when it's hot and humid.

It's also been known to grow in coffee machines and coffee grinds so if you have a coffee machine in your kitchen or compost coffee grinds in the open air you may be encouraging this mould to enter your environment.

*Please note there are no coffee grinds present in our Mushroom Grow Kit substrate*

While harmless to people and animals it's extremely fast growing and will overtake the mushroom mycelium and grow very fast.

What is looks like

Orange bread mould is bright neon orange. When it first appears it looks like fine orange wispy-ness which will develop into a bright orange powder-y looking patch. If left to grow it will make round orange lumpy formations. If you see it try to catch it at the wispy stage before it releases spores.

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What to do about it

If you encounter any orange bread mould, contain the source by sealing it in a plastic bag and dispose of it immediately.

It can appear and grow in as little as 8-12 hours and, if left unchecked, will take over your mushroom block and spread through the air to other incubating or fruiting mushroom blocks.

Don't disturb the orange bread mould as it will release a fine cloud of orange spores into the air.

Clean down any area that had orange bread mould nearby and look for a source such as coffee grinds, compost or damp untreated wood.

Cobweb mould


Cobweb mould is a common contaminant in mushroom growing and often occurs in later flushes. You might encounter this mould on a second flush for example.

Like the other moulds, it likes warm, high humidity environments.

Cobweb mould is often found on spoiled fruits and this can be a potential source which can spread to your mushrooms if in the same room. It can also develop on old mushroom stems so it's important to remove any stem butts from your first flush.

What it looks like

Cobweb mould looks like, you guessed it, cobwebs! It's fine a wispy or fluffy and a very light grey. It can be easily confused with mushroom mycelium except for its colour. Mycelium is bright white whereas cobweb mould is more grey.

How to identify contamination in mushroom growing (4)

What to do about it

  1. If you see any wispy cobweb mould forming around your mushrooms move your fruiting block to a colder area and stop misting it to see if this sudden change in conditions prevents it's growth.
  2. If it's just a small patch, carefully place a damp paper towel over the growth to contain any spores that have not yet released. You can then pour salt over the paper towel before gently removing any mushrooms that show symptoms of spotting.

Unfortunately it's more common that you will have to abandon your second flush if cobweb mould takes hold.

Don't eat any mushrooms which are touching cobweb mould.

Rhizopus stolonifer (black bread mould / pin head mould)


Pin head mould is one of the most common fungi in the world and you've probably seen it on rotting strawberries. Its a food decomposer and grows mostly in indoor environments. It's usually the first mould to appear on stale bread and also exists in soil and in the air.

Most commonly found in tropical and sub-tropical regions and more prevalent in summer, ideal conditions for this mould are between 25-30 degrees.

This fungi can be carried to your fruiting blocks by fungus gnats or fruit flies which are attracted to the smell of the mycelium and mushrooms.

What it looks like

Pin head mould can be easily confused with Cobweb mould. The way to tell them apart is the tiny black heads on the ends of the mycelium.

How to identify contamination in mushroom growing (5)

What to do about it

If you see any pin head mould forming around the mushrooms or on the substrate you will have to abandon this fruiting block.

This fungi can be dangerous to people with a weak immune system and cause illness. Don't inhale close to the mould or eat any mushrooms which have grown near this mould.

We hope you don't encounter any of these moulds on your mushroom growing adventures but if you do, don't stress! Fungi is all around us and they play vital roles in the interconnected systems we live in. Admire their ingenuity while you shake your fist at them and try again.

If you're growing a Mushroom Grow Kit purchased from us and it has developed some mould pleasesend us a message and we'll help you out in no time!

How to identify contamination in mushroom growing (2024)

FAQs

How to identify contamination in mushroom growing? ›

Off-odors: A strong, foul odor emanating from the growing containers or growing medium. Abnormal growth: Mushrooms that grow abnormally, such as those that are misshapen or stunted. Presence of molds: The appearance of molds, such as white or blue-green growths on the surface of the mushrooms or growing medium.

How to identify contamination in agar plates? ›

Visible Signs of Contamination

It will have the appearance of a white fuzziness originating from the area of inoculation. Molds, bacteria, and yeasts will appear on the plate in small spots but will spread quickly. Yeasts/bacteria will typically have a white/yellowish milky appearance.

How to tell the difference between mycelium and mold? ›

Healthy mycelium will be bright white and grow slower whereas the cobweb mold will be greyish-dull within a day and grows very quickly. All varieties of fungi in the Hypomyces group are parasites to other fungi. When left untreated the cobweb mold will cause your mushrooms to cease any healthy growth.

How to tell if liquid culture is contaminated? ›

Cloudiness in your liquid culture is a telltale sign of bacterial contamination. While some bacterial presence is common and manageable, excessive bacterial growth can lead to an overpowering takeover.

How to identify contamination? ›

So, although the threat of contamination from these microorganisms is ever-present, you can easily spot their presence by the turbidity of the growth medium or the floating, branching mycelia. Bacterial contamination can often be confirmed under a 10x microscope within a few days of contamination.

How to detect contaminants? ›

Raman spectroscopy, fluorescence microscopy, optical microscopy and also ATR-FTIR spectroscopy can provide molecular composition information of organic materials, and help to identify unknowns.

How can you quickly identify microbial contamination? ›

Celsis® rapid microbial detection methods provide objective quality control results in half the time compared to traditional testing methods, allowing your QC microbiology team to quickly confirm the presence or absence of microbial contamination in samples using an automated, reagent-based assay.

How will you identify the media of contamination? ›

If your media contains phenol red: look for changes in the color of your media as this indicates pH changes. If it starts to go orange/yellow, you may have a problem (either contamination or you need to replenish your cell's media supply more frequently). Look for signs of turbidity or cloudiness of the media.

How can you identify bacteria and fungi on agar plates? ›

Upon these agar mediums, bacteria manifest as microscopically tiny oily spot formations. Conversely, fungi manifest as a powdery mat spreading across the entire surface of the agar plate. A primary distinction between bacterial and fungal colonies arises from their origins.

How to prevent mushroom contamination? ›

For the vast majority of grow kits mould will never be a problem for you but here are a few tips to avoid it.
  1. Avoid warm humid weather. ...
  2. Avoid soils and plants. ...
  3. Clean your knife or cutting implement. ...
  4. Avoid touching the substrate and plastic with your fingers. ...
  5. Avoid over-watering. ...
  6. Open your kit as soon as you receive it.
Sep 26, 2021

What does mycelium growth look like on agar? ›

If you cultivate mushroom mycelium on agar medium there will appear different forms of mycelium. The two main forms are: rhizomorph mycelium and 'fluffy' mycelium (looks like cotton). For further cultivation and introduction of fruiting only the rhizomorph mycelium is suitable.

How to tell if mycelium is contaminated? ›

While mycelium is typically white and filamentous, contamination generally takes the form of green, blue, gray, or black patches or discolorations in your substrate.

What does a contaminated culture look like? ›

Culture media contaminated with bacteria (left) appear turbid and yellow whereas non-contaminated media (right) appear clear and red.

How to detect fungal contamination? ›

For bacteria detection, use soybean–casein digest agar medium and for fungi detection, use Sabouraud glucose agar media, to which antibiotic has previously been added. After the agar solidifies, incubate at least for 5 days at 30–35 °C for bacteria detection and at 20–25 °C for fungi detection.

How do you check if a mushroom is safe? ›

Avoid mushrooms with white gills, a skirt or ring on the stem and a bulbous or sack like base called a volva. You may be missing out on some good edible fungi but it means you will be avoiding the deadly members of the Amanita family. Avoid mushrooms with red on the cap or stem.

How to identify contamination in broth culture? ›

Bacterial, fungal (including molds), and yeast contamination are usually visible to the unaided eye as rapid-onset turbidity and color change of the culture medium (provided that the medium is supplemented with phenol red, the most common non-toxic pH indicator).

How do you identify microbiological contamination? ›

Detection of microorganisms in foods

Bacterial toxins in food and beverage samples can be analyzed with various test kits, including ELISA assays, real-time PCR tests, nutrient plates, and agar plates. For detecting pathogenic bacteria, immunological methods (ELISA) are available.

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