FAQs
The method of sterilization I am talking about is known as Tyndallization, fractional sterilization or discontinuous heating. The process simply involves boiling the goods to be sterilized in a jar at 100°C for about 15 to 20 minutes per day, for three days in a row.
Which method is used for sterilizing liquids? ›
Microbial growth media and other liquids used for working with microbes must be sterilized to prevent contaminants from growing in them. The most effictive way to sterilize liquids is to heat them in an autoclave or a pressure cooker.
What can be used in place of autoclave for sterilization? ›
Dry heating remains an important sterilisation option due to the some items being immune to the effects of an autoclave, making this method a useful backup for all labs and research facilities.
What are the methods of sterilization other than autoclave? ›
Table 59.2
| Methods of Sterilization | Example |
|---|
| High temperature | Steam, dry heat |
| Low temperature | Ethylene oxide gas, hydrogen peroxide, ozone, gas plasma, gaseous chlorine dioxide, ionizing radiation, pulsed light |
| Liquid chemicals | Chemical sterilants |
| Others | Filtration |
I use 10 grams agar 10 grams light yeast and 1 grams nutritional yeast. Mix everything in mason jar, submerge in boiling water for two hours. Then when it's cool enough I pour in a sab after sterilising everything with 70% alcohol.
Can you sterilise with just boiling water? ›
Sterilising by boiling
Make sure the items you want to sterilise in this way are safe to boil. Boil the feeding equipment in a large pan of water for at least 10 minutes, making sure it all stays under the surface. Set a timer so you do not forget to turn the heat off.
How do you sterilize medical equipment without an autoclave? ›
Medical devices are sterilized in a variety of ways including using moist heat (steam), dry heat, radiation, ethylene oxide (EtO) gas, vaporized hydrogen peroxide, and other sterilization methods (for example, chlorine dioxide gas, vaporized peracetic acid, and nitrogen dioxide).
How do you sterilize bottles without a pressure cooker? ›
Fill the pot with water until it covers the jars and bottles by 1 inch (2.5 cm). Boil the jars and bottles. Bring the water to a full, rolling boil. If you're at an altitude of less than 1,000 feet (304.8 m), boil them for 10 minutes.
What are the 7 steps of sterilization? ›
There are seven primary methods for medical device sterilization. These methods are steam sterilization, radiation sterilization, dry heat sterilization, sterilization by filtration, gas sterilization (such as ethylene oxide sterilization), vapor sterilization, and liquid sterilization.
Which method is most commonly used for sterilization? ›
Wet Heat (Autoclaving)
Autoclaving is the most popular method of lab sterilization. This process uses pressurized steam to heat the item that requires sterilization. Autoclaving is an incredibly effective procedure. It will effectively kill all microbes, spores and viruses.
Autoclaves are widely used for heat sterilisation and commonly use steam heated to 121–134 °C (250–273 °F) with a holding time of at least 15 minutes at 121 °C or 3 minutes at 134 °C, longer for liquids and surgical instruments packed in layers of cloth.
How do you sterilize glassware without an autoclave? ›
The most effective and used method of sterilization of glassware is dry heat treatment using hot air oven. On exposing the glassware to dry heat, the inhibition in growth occurs due to denaturation of proteins and oxidation of biomolecules.
What is the substitution of autoclave? ›
If you do not need a really restrictive control on pressure for the autoclave, you can try to substitute it by a pressure cooker.
How do you sterilise without a machine? ›
Sterilising by boiling
Boil the feeding equipment in a large pan of water for at least 10 minutes, making sure it all stays under the surface. Set a timer so you do not forget to turn the heat off. Remember that teats tend to get damaged faster with this method.
How do you sterilise something at home? ›
Sterilising by boiling
Never leave hot pans and liquids unattended. Make sure that whatever you sterilise in it is safe to boil. Boil the feeding equipment in water for at least 10 minutes, making sure that all items stay under the surface of the water. You may need to use a small plate to keep bottles in the water.
What is the quickest way to sterilize solution without heating? ›
Filtration is the fastest way to sterilize a solution without heating. The pore size used in this method is too small to filter microorganisms. Filters with a pore size of 0.2 µm are usually used to remove bacteria. Membrane filters are more commonly used than sintered filters or Seitz filters or candle filters.
How do you self sterilise? ›
Sterilise using one of the following methods: steam sterilising (electric or microwave according to manufacturers instructions), boiling in water for at least 5 minutes or liquid sterilising solution. After boiling or sterilising allow all parts to cool.
